Effects of DEHP on embryonic development and DNA damage of Pinctada maetensii
ZHAO Chun-feng1,3, DIAO Xiao-ping1,3, CAO Jia1, LI Ping2, ZHENG Peng-fei1, SONG Qin-qin1, ZHOU Hai-long1,3, WANG Hai-hua1
1. College of Agriculture, Hainan University, Haikou 570228, China;
2. College of Environment and Plant Protection, Hainan University, Haikou 570228, China;
3. Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou 570228, China
Abstract：Di-2-ethylhexyl phthalate (DEHP) is used as a plasticizer commonly and exists widely in environment.The effects of early D-shaped veliger larvae development and embryonic DNA damage of Pinctada martensii exposure to DEHP were studied in this report.The results showed that DEHP had a significant effect on the development of D-shaped veliger larvae.There was a positive correlation between mortality rate of D-shaped veliger larvae and exposure time of DEHP in a same concentration group.In the concentration groups of (4~32 )mg/L,there was a significant dose-response relationship between the larval mortality rate and exposure concentrations at different exposure time.It can be concluded that DEHP exposure caused adverse effects on the development of D-shaped veliger larvae and may also influence the population structure of Pinctada martensii.DEHP exposure induced DNA damage of embryo cells significantly.There was a good dose-effect relationship between DNA migration and DEHP concentrations.Tail length (TL),tail DNA content (T-DNA) and olive tail moment (OTM) increased significantly with exposure concentration.The results indicated that developmental and morphological changes of D-shaped veliger larvae and the DNA damages in embryo cells of Pinctada martensii could be used as sensitive biomarkers of DEHP exposure in the marine environment.
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