1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
2. Colloge of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China
Abstract：The gene expression patterns of P4501A1, P-gp and HSP70 of Portunus trituberculatus were both measured using realtime fluorescence quantitative PCR during crab exposed to environmentally relevant concentration of Benzo[a]pyrene (0.05, 0.15, 0.45 μg/L, respectively) and the release of the test.The results showed that P451A1, P-gp and HSP70 mRNA could be induced, besides, the content of P4501A1 mRNA induced is more significantly(P<0.05).The content of three genes mRNA were decrease markedly after the release of the test and the P4501A mRNA has more obvious changes.In a word, The gene expression of P4501A1, P-gp and HSP70 were increase at the beginning of Benzo[a]pyrene exposure, and then decreased after the release of the test.The above data suggest that Benzo[a]pyrene could induced high concentration of P451A1 mRNA in the liver of crab to monitor indicates contamination of organic pollutants in the water of PAHs.
XU Gao-peng,JIANG Mei,LI lei et al. Effect of Benzo[a]pyrene on the P4501A1,P-glycoprotein and HSP70 expression in Portunus trituberculatus[J]. Marine Environmental Science, 2015, 34(5): 647-653.
LEVINE S L,ORIS J T.CYP1A expression in liver and gill of rainbow trout following waterborne exposure:implications for biomarker determination[J].Aquatic Toxicology,1999,46(3/4):279-287.
COSTA J,REIS-HENRIQUES M A,WILSON J M,et al.P-glycoprotein and CYP1A protein expression patterns in Nile tilapia (Oreochromis niloticus) tissues after waterborne exposure to benzo(a)pyrene (BaP)[J].Environmental Toxicology and Pharmacology,2013,36(2):611-625.
[an error occurred while processing this directive] | [an error occurred while processing this directive]
