1. School of Marine Sciences, Ningbo University, Ningbo 315000, China;
2. Key Laboratory South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510000, China
Abstract：To evaluate the effects of carbohydrate addition on the artificial substrate biofilm, we studied dynamics of bacterial community structures on the biofilms of artificial substrates(bolting-silk nets) in six Litopenaeus vannamei aquaculture ponds by using methods of bacterial plate coating and terminal restriction fragment length polymorphism (T-RFLP).The results of bacterial plate coating showed that, during the 30 days' experiment, the heterotrophic bacteria on the biofilm of the two groups maintain relatively stable when the biofilm matured.The quantity of Vibrio in carbon regulation group was decreased significantly by 91.85% compared with control group (p<0.01), the number ratio between Vibrio and heterotrophic bacteria was 96.98% lower than that of control group (p<0.01).The T-RFLP profiles showed that the relative abundance of T-RF of 126、432、434、436、485、487、488 bp was significantly changed.Further analysis indicated that the bacterial abundance and diversity increased 100% and 33.9%, respectively, on the biofilms of carbon regulation groups, compared with those of the control groups.The moving window analysis showed that the community change rates in carbon regulation groups and control groups were 12.4%±0.3% and 23.2%±26.38%, respectively.The results obtained in the present study demonstrated that carbon regulation might not only increase the number of heterotrophic bacteria which inhibited the proliferation of Vibrio cholera by competition, but also improve the stability of bacterial community structure on the biofilms of artificial substrates in aquaculture ponds.
ZHANG Zhen-zhen,DONG Hong-biao,DUAN Ya-fei et al. Effects of carbohydrate addition on dynamics of bacterial community structures on the biofilms of artificial substrates[J]. Marine Environmental Science, 2015, 34(5): 686-691.
UJIIÉ H,HATAKEYAMA Y,GU X X,et al.Upward decrease of organic C/N ratios in the Okinawa Trough cores:proxy for tracing the post-glacial retreat of the continental shore line[J].Palaeogeography,Palaeoclimatology,Palaeoecology,2001,165(1/2):129-140.
WANG H J,YANG Z S,SAITO Y,et al.Stepwise decreases of the Huanghe (Yellow River) sediment load (1950-2005):Impacts of climate change and human activities[J].Global and Planetary Change,2007,57(3/4):331-354.
[9]
HE B Y,DAI M H,ZHAI W D,et al.Distribution,degradation and dynamics of dissolved organic carbon and its major compound classes in the Pearl River estuary,China[J].Marine Chemistry,2010,119(1/2/3/4):52-64.
[10]
FAN H,HUANG H J.Response of coastal marine eco-environment to river fluxes into the sea:A case study of the Huanghe (Yellow) River mouth and adjacent waters[J].Marine Environmental Research,2008,65(5):378-387.
[11]
NI H G,LU F H,LUO X L,et al.Riverine inputs of total organic carbon and suspended particulate matter from the Pearl River Delta to the coastal ocean off South China[J].Marine Pollution Bulletin,2008,56(6):1150-1157.
[12]
REN L L,WANG M R,LI C H,et al.Impacts of human activity on river runoff in the northern area of China[J].Journal of Hydrology,2002,261(1/2/3/4):204-217.
[13]
DAI M,WANG L,GUO X,et al.Nitrification and inorganic nitrogen distribution in a large perturbed river/estuarine system:the Pearl River Estuary,China[J].Biogeosciences,2008,5:1227-1244.
WANG H J,YANG Z S,SAITO Y,et al.Interannual and seasonal variation of the Huanghe (Yellow River) water discharge over the past 50 years:Connections to impacts from ENSO events and dams[J].Global and Planetary Change,2006,50(3/4):212-225.
[16]
CALLAHAN J,DAI M H,CHEN R F,et al.Distribution of dissolved organic matter in the Pearl River estuary,China[J].Marine Chemistry,2004,89(1/2/3/4):211-224.
WANG H J,YANG Z S,WANG Y,et al.Reconstruction of sediment flux from the Changjiang (Yangtze River) to the sea since the 1860s[J].Journal of Hydrology,2008,349(3/4):318-332.
[an error occurred while processing this directive] | [an error occurred while processing this directive]
