Abstract:The distribution and composition of total hydrolyzed amino acids (THAA), dissolved combined amino acids (DCAA) and dissolved free amino acids (DFAA) were analyzed in the seawater samples collected at 43 stations located in the East China Sea and the Yellow Sea in March 2011. The results showed that the average concentration of THAA in the surface water was 2.98±1.72 μmol/L (in the range of 1.27 to 8.54 μmol/L), while the average concentrations of DCAA and DFAA in the surface water were 2.76±1.63 μmol/L (in the range of 0.91 to 7.70 μmol/L) and 0.31±0.21 μmol/L (in the range of 0.11 to 1.14 μmol/L), respectively. The concentrations of dissolved amino acids displayed a decreasing trend from inshore to offshore stations, especially DCAA and THAA. In the vertical distribution, the high values of THAA appeared in the subsurface layer and in the bottom layer of the water column. In the study area, major constituents of dissolved amino acids were aspartic, glycine, serine, glutamic acid and alanine acid. Correlation matrix analysis of amino acids indicated that 5 pairs of amino acids in DFAA showed significant positive correlations. No significant correlations were found between the concentrations of dissolved amino acids and environmental factors such as chlorophyll a, dissolved organic carbon (DOC) and dissolved inorganic nitrogen (DIN).
WANG Peng,CHEN Yan,YANG Gui-peng et al. Distribution and composition of dissolved amino acids in the East China Sea and the Yellow Sea during spring[J]. Marine Environmental Science, 2015, 34(2): 217-224.
SHARP J H. The distributions of inorganic nitrogen and dissolved and particulate organic nitrogen in the sea[J]. Nitrogen in the Marine Environment, 1983:1-35.
[2]
DITMAR T, FITZNAR H P, KATTNER G. Origin and biogeochemical cycling of organic nitrogen in the eastern Arctic Ocean as evident from D-and L-amino acids[J]. Geochimica et Cosmochimica Acta, 2001, 65(22):4103-4114.
[3]
BURDIGE D J. Microbial processes affecting alanine and glutamic acid in anoxic marine sedimentsY[J]. FEMS Microbiology Ecology, 1991, 85:211-232.
[4]
VEUGER B, MIDDELBURG J J, BOSCHKER H T S, et al. Microbial uptake of dissolved organic and inorganic nitrogen in Randers Fjord. Estuarine[J]. Coastal and Shelf Science, 2004, 61(3):507-515.
[5]
MIDDELBOE M, BORCH N, KIRCHMAN D. Bacterial utilization of dissolved free amino acids, dissolved combined amino acids and ammonium in the Delaware Bay estuary: effects of carbon and nitrogen limitation[J]. Marine Ecology Progress Series Oldendorf, 1995, 128(1):109-120.
DANIELA U, VENUGOPALAN I, PETRA S, et al. Biogeochemistry of particulate organic matter from the Bay of Bengal as discernible from hydrolysable neutral carbohydrates and amino acids[J]. Marine Chemistry, 2005, 96:155-184.
[8]
MATSUMOTO K, UEMATSU M. Free amino acids in marine aerosols over the western North Pacific Ocean[J]. Atmospheric Environment, 2005, 39(11):2163-2170.
[9]
YANG G P, CHEN Y, GAO X C. Distribution of dissolved free amino acids, dissolved inorganic nitrogen and chlorophyll a in the surface microlayer and subsurface water of the Yellow Sea, China[J]. Continental Shelf Research, 2009, 29(14):1737-1747.
GARRETT, W D. Collection of slick-forming materials from the sea surface[J]. Limnology and Oceanography, 1965(10):10, 602-605.
[13]
LINDROTH P, MOPPER K. High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with O-phthaldialdehyde[J]. Analytical Chemistry, 1979, 51(11): 1667-1674.
[14]
PARSONS T R, MAITA Y, LALLI C M. A Manual for Chemical and Biological Methods for Seawater Analysis[M]. Oxford: Pergamon Press, 1984:107-109.
[15]
YAMASHITA Y, TANOUE E. Distribution and alteration of amino acids in bulk DOM along a transect from bay to oceanic waters[J]. Marine Chemistry, 2003, 82(3/4):145-160.
[16]
GARRASI C, DEGENS E T, MOPPER K. The free amino acid composition of seawater obtained without desalting and preconcentration[J]. Marine Chemistry, 1979, 8(1):71-85.
BRAWEN J, BUTLER E I, CHAPMAN J, et al. Changes in dissolved free amino acid composition in sea water associated with phytoplankton populations[J]. The science of the Total Environment, 1995, 172:145-150.
LISS P S. DUCE R. The Sea Surface and Global Change[M]. NewYork:Cambridge University Press, 1997:534-550.
[22]
YANG G P, TSUNOGAI S. Biogeochemistry of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) in the surface microlayer of the western North Pacific[J]. Deep-Sea Research I, 2005, 52:553-567.
[23]
LIEBEZEIT G, BÉLTER M. Distribution of particulate amino acids in the Bransfield Strait[J]. Polar Biology, 1986, 5(4):199-206 .
[24]
LEE C, BADA J. Dissolved amino acids in the equatorial Pacific, the Sargasso Sea, and Biscayne Bay[J]. Limnology and Oceanography, 1977, 22(3):502-510.
[25]
HENRICHA S M, FARRINGTON J W. Amino acids in interstitial waters of marine sediments[J]. Nature, 1979, 279:319-322.
[26]
YAMASHITA Y, TANOUE E. Distribution and alteration of amino acids in bulk DOM along a transect from bay to oceanic waters[J]. Marine Chemistry, 2003, 82(3/4):145-160.