Toxic effect of nonylphenol (NP) on the mantle of Paphia undulate
GONG Xiu-yu1,2, HUANG Zhi-fei1,3, WANG He-wei1,3, ZHANG Zhe1, MA Sheng-wei1, CHEN Hai-gang1, CAI Wen-gui1
1. The Ministry of agriculture fishery resources and Environmental Sciences observation experiment station, Key Laboratory of Guangdong Province fishery ecological environment, South China Sea Fisheries Research China Inst, Guangzhou 510300, China;
2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
3. College of Marine Science, Shanghai Ocean University, Shanghai 201306, China
Abstract：A laboratory experiment was carried out to study the 96 h LC50 of nonylphenol (NP) to Paphia undulate. Acute toxic experiment showed the 96 h LC50 of NP to Paphia undulate was 0.26 mg/L. The changing tendency of superoxide dismutase (SOD)activity, peroxidase (POD) activity, glutathione (GSH)content and malondialdehyde (MDA) content which exist in P. undulata's mantle were simultaneously observed when P. undulate was exposed to low, middle and high concentration of NP (1, 10 and 25 μg/L) and after the stress was released. The results show that: after Stress Day one, SOD activity of the mantle of P. undulate was slightly inhibited only at low concentration, then showed a trend of first and then decreased. The activity of POD was only inhibited at the time of Day 15 after exposing to low, medium concentration NP group, but each concentration group were almost induced at the other time. The content of GSH on Day one and Day seven were basically lower than the control group, but on Day 15 the content of GSH was significantly higher than that of control group at three different concentrations(P < 0.01).The content of MDA was increased significantly with the prolonging stress time. After the release with clear water, SOD activity and POD activity, GSH content returned to normal level in the low concentration group (1 μg/L); only the content of GSH had a better restoring in the middle and high concentration groups (10, 25 μg/L). This study shows that NP has marked oxidative damage to P. undulate's mantle, and the damage increased with the increasing concentration of NP. SOD activity, POD activity of the mantle, MDA content and GSH content basically cannot recover to the control level of high concentration of NP stress released after the experiment.
GONG Xiu-yu,HUANG Zhi-fei,WANG He-wei et al. Toxic effect of nonylphenol (NP) on the mantle of Paphia undulate[J]. Marine Environmental Science, 2015, 34(2): 234-239.
BEJA O, SUZUKA M T, HELDELBERG J F, et al.Unsuspected diversity among marine aerobic anoxygenic phototrophs[J].Nature, 2002, 415:630-633.
[2]
LEE H B, PEART T E, CHART J, et al.Occurrence of endocrine-disrupting chemicals in sewage and sludge samples in Toronto, Canada [J].Water Quality Research Journal of Canada, 2004, 39 (1):57-63.
[3]
KOLBER Z S, PLUMLEY F G, LANG A S, et al.Contribution of aerobic photoheterotrophic bacteria to the carbon cycle in the ocean[J].Science, 2001, 292 (5526):2492-2495.
ISOBE T, NISHIYAMA H, NAKASHIMA A, et al.Distribution and behavior of nonylphenol, octylphenol, and nonylphenol monoethoxylate in Tokyo metro Politan area:their association with aquatic Particles and sedimentary distributions [J].Environmental Science & Technology, 2001, 35(6):1041-1049.
[9]
Zhou J, BURUNS M N, and TIEJDE J M.DNA recovery from soils of diverse composition[J].Appl Environ Microbiol, 1996, 62:316-322.
[10]
PETRVIC M, LACORTE S, PAULA Vianab, et al.Pressurized liquid extraction followed by liquid chromatography-mass spectrometry for the determination of allkylphenolic compounds in river sediment [J].Journal of Chromatography A, 2001, 959(1/2):15-23.
QIU X Y, Wu L Y, Huang H S.Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning[J].Applied and Environmental Microbiology, 2001, 67:880-887.
[15]
EKELUND, BERGMAN, GRANMO, et al.Bioaccumulation of 4-nonylphenol in marine animals A reevaluation [J].Environmental Pollution, 1990, 64(2):107-120.
GRUDEN C, SKERLOS S, ADRIAENS P.Flow cytometry for microbial sensing in environmental sustainability applications:current status and future prospects[J].Microbial Ecol, 2004, 49:37-49.
[22]
CALABRESE E J.Hormesis:changing view of the dose-response, a personal account of the history and current status [J].Mutation Research, 2002, 511:181-189
SCHWALBACH M S, FUHRMAN J A.Wide-ranging abundances of aerobic anoxygenic phototrophic bacteria in the world ocean revealed by epifluorescence microscopy and quantitative PCR[J].Limnol Oceanogr, 2005, 50(2):620-628.
LZAWA, INOUE Y, KIMURA A.Oxidative stress response in yeast:effect of glutathione on adaptaion to hydrogen peroxide stess in Sacchar [J].FEBS Letters, 1995, 368(1):73-76.
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