Distribution and diversity of culturable bacteria in soils and sediments from Ny-Alesund,Arctic
SU Jie1,2, HAN Jun-li1,3, MING Hong-xia1,2, ZHAO Xiao-hui1,3, SHI Yan1,2, GUAN Dao-ming1,2, FAN Jing-feng1,2
1. National Marine Environmental Monitoring Center, Dalian 116023, China;
2. The Ecological Environment Key Laboratory of Coastal Water of the State Oceanic Administration, Dalian 116023, China;
3. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
Abstract：With the aim to study the distribution and diversity of culturable bacteria in Arctic,two surface soil samples and eight sediment samples were collected from Ny-Alesund and analyzed using the culture-based method and 16S rRNA gene sequence.A total of 343 isolates from 10 stations were characterized according to morphological character of bacterial colony.Molecular identification and phylogenetic analysis demonstrated that 47 representative strains were grouped into 4 phylum,6 classes,18 genera and 29 species.According to the taxonomy,bacillis were grouped into 6 genera with a high diversity.The γ-Proteobacteria were the dominant bacterial lineage,belonging to 13 species.The bacterial diversity and community composition among marine sediments,lake sediments,river sediments and soils displayed spatial variety.Bacterial diversity was most abundant in marine sediments and subsequent in lake sediments.
SU Jie,HAN Jun-li,MING Hong-xia et al. Distribution and diversity of culturable bacteria in soils and sediments from Ny-Alesund,Arctic[J]. Marine Environmental Science, 2018, 37(2): 274-280,293.
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