Effects of pretreatment methods on the extraction amount and the stable carbon isotopic composition of marine microalgae fatty acid
LIU Yu1, YAO Jing-yuan1, LI Ying2, ZHANG Xu-feng1
1. College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China;
2. Environmental Information Institute, Dalian Maritime University, Dalian 116026, China
Abstract：To explore the effects of different pretreatments on the extraction amount and carbon stable isotope ratio (δ13C) of fatty acid in Marine microalgae,the relative content and δ13C values of the products of fatty acid methyl esters(FAMEs) were measured by GC/MS and GC/IRMS under the different acid catalytic condition,and then were compared and analyzed.The results show that hydrochloric acid catalysis is more suitable for fresh samples of microalgae than sulfuric acid in the process of producing polyunsaturated FAMEs; When sulfuric acid was used as catalysis,the relative contents and δ13C values of FAMEs also exist certain differences in different pretreatments (A-fresh samples of microalgae,B-dry processing,C-solvent extraction) of the microalgae samples.
LIU Yu,YAO Jing-yuan,LI Ying et al. Effects of pretreatment methods on the extraction amount and the stable carbon isotopic composition of marine microalgae fatty acid[J]. Marine Environmental Science, 2015, 34(3): 457-461.
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