Abstract: The distributed hydrological model soil and water assessment tool(SWAT) of the Biliu river basin was built by using multi-source comprehensive data, and the model was calibrated and validated.The results show that runoff simulation in the Biliu river basin is mostly effective and the uncertainty of the model is slight supporting by the established SWAT model.In the calibration period, the Nash-Sutcliffe efficiency coefficient(Ens), the determination coefficient(R²) and the relative deviation(PBIAS) are 0.80, 0.86 and 18.9% correspondingly, the model uncertainty P factor and R factor are 0.78 and 0.58 respectively.In the validation period, Ens, R² and PBIAS value are 0.87, 0.88 and 1.9% respectively.As results, building of the distributed hydrological model can solve the problem of limited monitoring rivers and difficulty of obtaining data of the runoff into the sea in marine scientific research work, so to provide high resolution flow data for assessments.