原油分散液对小新月菱形藻C、N稳定同位素组成的影响

doi:10.13634/j.cnki.mes20150405

摘要
参考文献
相关文章

全文: PDF (1231 KB) HTML ( ) 输出: BibTeX | EndNote (RIS) 背景资料

摘要以微藻类小新月菱形藻(Nitzschia closterium)为研究对象,通过设置不同的原油分散液(WAF)浓度,分别进行了急性(4 d)和亚急性(29 d)毒理实验;通过细胞计数、丙二醛(MDA)测定、稳定同位素组成分析等方法测定了原油分散液(WAF)对小新月菱形藻生长曲线、C和N稳定同位素组成(δ¹³C、δ¹⁵N值)的影响。结果表明,WAF在低浓度(≤1.0 mg/L)长期作用下对小新月菱形藻的生长有促进作用,而较高浓度(≥5.0 mg/L)则使藻细胞生长受到抑制;小新月菱形藻的丙二醛含量随时间及WAF浓度增大而逐渐增加,最高可达1.65倍。WAF在低浓度(≤1.0 mg/L)作用下,藻细胞的δ¹³C、δ¹⁵N值都趋于增大,而较高浓度(≥5.0 mg/L)则使藻细胞的δ¹³C、δ¹⁵N值趋于变小,这表明,微藻类的C、N稳定同位素组成能够从一定程度上反映污染程度,因此可作为海洋溢油污染监测的一种新指标。

	服务

	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	刘瑀
	李颖
	王晓琦
	王海霞
	韩俊松

关键词：原油小新月菱形藻丙二醛稳定同位素

Abstract：This study made acute (4 d) and chronic (29 d) experiments by setting different oil concentrations (WAF) and using Nitzschia closterium as the research object.We measured the effects of WAF on N.closterium by cell counting,malondialdehyde (MDA) measuring and the carbon/nitrogen stable isotopic analyses.The results show that WAF stimulated the growth of N.closterium in low concentration (≤1.0 mg/L),while the growth of N.closterium was restrained in relatively high concentrations (≥5.0 mg/L).The MDA content increased with the growth time and the increase of WAF concentration,up to the maximum of 1.65 times.The δ¹³C and δ¹⁵N value was higher in low concentration (≤1.0 mg/L),while lower in relatively high concentration (≥5.0 mg/L).Therefor the stable isotopic composition of microalgae may be used as an alternative method for oil pollution monitoring.

Key words： crude oil Nitzschia closterium malondialdehyde stable isotopic composition

收稿日期: 2014-09-28;

PACS:

基金资助:

国家海洋公益项目(2012418012-02)

作者简介:刘瑀(1965-),男,教授,陕西西安人,主要从事海洋环境化学领域的研究,E-mail:ylsibo@foxmail.com

引用本文:

刘瑀,李颖,王晓琦等. 原油分散液对小新月菱形藻C、N稳定同位素组成的影响[J]. 海洋环境科学, 2015, 34(4): 503-507.

LIU Yu,LI Ying,WANG Xiao-qi et al. Effect of crude oil dispersion on carbon and nitrogen stable isotopic composition of Nitzschia closterium[J]. Marine Environmental Science, 2015, 34(4): 503-507.

链接本文:

http://hyhjkx.nmemc.org.cn/CN/10.13634/j.cnki.mes20150405 或 http://hyhjkx.nmemc.org.cn/CN/Y2015/V34/I4/503

References

[1]	王君丽,刘春光,冯剑丰,等.石油烃对海洋浮游植物生长的影响研究进展[J].环境污染与防治,2011,33(4):81-86.
[2]	黄逸君,江志兵,曾江宁,等.石油烃污染对海洋浮游植物群落的短期毒性效应[J].植物生态学报,2010,34(9):1095-1106.
[3]	赵文.水生生物学[M].北京:中国农业出版社,2005:515-518.
[4]	李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:214-217.
[5]	GALTSOFF P S,PRYTHERCH H F,SMITH R O,et al.The effects of crude oil pollution on oysters in Louisiana waters[J].The Scientific Monthly,1935,43(1):121-129.
[6]	张蕾,王修林,韩秀荣,等.石油烃污染物对海洋浮游植物生长的影响——实验与模型[J].青岛海洋大学学报:自然科学版,2002,32(5):804-810.
[7]	GILDE K,PINCKNEY J L.Sublethal effects of crude oil on the community structure of estuarine phytoplankton[J].Estuaries and Coasts,2012,35(3):853-861.
[8]	陶功华.低剂量兴奋效应作用机制的研究进展[J].中山大学研究生学刊:自然科学、医学版,2007,28(1):16-21.
[9]	田丹,赵文,王媛,等.镉胁迫对两种海洋微藻生长和抗氧化系统的影响[J].大连海洋大学学报,2010,25(5):417-427.
[10]	林冬,朱诚,孙宗修.镉敏感水稻突变体在镉胁迫下活性氧代谢的变化[J].环境科学,2006,27(3):561-566.
[11]	王海霞,刘瑀,关春江,等.营养条件对微藻碳、氮稳定同位素组成的影响[J].中国环境科学,2014,34(3):727-733.
[12]	VUORIO K,MEILI M,SARVALA J.Taxon-specific variation in the stable isotopic signatures (δ¹³C and δ¹⁵N) of lake phytoplankton[J].Freshwater Biology,2006,51(5):807-822.
[13]	APRAIZ I,CAJARAVILLE M P,CRISTOBAL S.Peroxisomal proteomics:Biomonitoring in mussels after thePrestige's oil spill[J].Marine Pollution Bulletin,2009,58(12):1815-1826.
[14]	WU Y Y,XU Y,LI H T,et al.Effect of acetazolamide on stable carbon isotope fractionation in Chlamydomonas reinhardtii and Chlorella vulgaris[J].Chinese Science Bulletin,2012,57(7):786-789.
[15]	洪义国.硝酸盐氮氧稳定同位素分馏过程记录的海洋氮循环研究进展[J].地球科学进展,2013,28(7):751-764.
[16]	NEEDOBA J A,HARRISON P J.Influence of low light and a light:Dark cycle on NO_3- uptake,intracellular NO_3-,and nitrogen isotope fractionation by marine phytoplankton[J].Journal of Phycology,2004,40(3):505-516.
[17]	NEEDOBA J A,SIGMAN D M,HARRISON P J.The mechanism of isotope fractionation during algal nitrate assimilation as illuminated by the ¹⁵N/¹⁴N of intracellular nitrate[J].Journal of Phycology,2004,40(3):517-522.
[18]	沈国兴,严国安,彭金良,等.农药对藻类的生态毒理学研究II:毒性机理及其富集和降解[J].环境科学进展,1999,7(6):131-138.