Abstract:Intact sediment cores from the Yellow River were studied for denitrification rate (De) based on benthic fluxes of molecular nitrogen(N2) by a laboratory incubation study; meanwhile correlation between sediments De and concentration of dissolved organic carbon (DOC) and nitrate,and sediment oxygen consumption rate (SOC) was discussed.The mean value of De for the control treatment was 14.81±9.43 μg N/m2/h.For the extragenousnitrogen addition treatment,the highest De was investigated in N1 level (4.5 mg/L) with a mean value of 21.99±12.45 μg N/m2/h.For the extragenouscarbon addition treatment,the highest De was investigated in C1 level (2.4 mg/L) with a mean value of 24.33±14.38 μg N/m2/h.In theory,sediment denitrification rate up to peak,when concentration of nitrate and DOC in overlying water was 9.8 and 4.0 mg·L-1 respectively; the optimal C/N for sediment denitrification was 2.3.Correlation between De and SOC showed different patterns depending on nitrate and DOC level.Our study investigated that De was significant positively related to SOC under lower nitrate and DOC level,while negativelyrelated to SOC under higher DOC and lower nitrate level; however,no significant correlation between De and SOC was investigated when overlying water showed higher nitrate and lower DOC level.
YANG Li-biao,LEI Kun,MENG Wei et al. Impact of C and N concentrations on sediments denitrification rate of the Yellow River[J]. Marine Environmental Science, 2016, 35(6): 868-875,881.
OMNES P,SLAWYK G,GARCIA N,et al.Evidence of denitrification and nitrate ammonification in the River Rhone plume (northwestern Mediterranean Sea)[J].Marine Ecology Progress Series,1996,141:275-281.
[4]
COSTANZAR,D'ARGE R,DE GROOT R,et al.The value of the world's ecosystem services and natural capital[J].Nature,1997,387(6630):253-260.
[5]
Millennium Ecosystem Assessment (MA).Ecosystems and human well-being:a frameworkfor assessment[M].2nd ed.Washington DC:Island Press,2003.
GRASSHOFF K,EHRHARDT M,KREMLING F.Methods of seawater analysis[M].3rd ed.Weinheim:Wiley-VCH Verlag GmbH,1999:27-228.
[9]
BANGE H W. Global change:it's not a gas[J]. Nature,2000,408(6810):301-302.
[10]
KANG S W,OH B C,PARK K S,et al.Near-field dilution of wastewater effluents discharged from submerged ocean outfalls in Masan Bay[J].KSCE Journal of Civil Engineering,1999,3(4):395-405.
[11]
ARMSTRONG F A J,BOALCH G T.The ultra-violet absorption of sea water[J].Journal of the Marine Biological Association of the United Kingdom,1961,41(3):591-597.
[12]
LAURSEN A E,SEITZINGER S P.Diurnal patterns of denitrification,oxygen consumption and nitrous oxide production in rivers measured at the whole-reach scale[J].Freshwater Biology,2004,49(11):1448-1458.
[13]
BROWN M A,CLARKSON B D,BARTON B J,et al.Ecological compensation:an evaluation of regulatory compliance in New Zealand[J].Impact Assessment and Project Appraisal,2013,31(1):34-44.
[14]
LIU S M,LI R H,ZHANG G L,et al.The impact of anthropogenic activities on nutrient dynamics in the tropical Wenchanghe and Wenjiaohe Estuary and Lagoon system in East Hainan,China[J].MarineChemistry,2011,125(1/2/3/4):49-68.
RAVISHANKARA A R,DANIEL J S,PORTMANN R W. Nitrous oxide (N2O):the dominant ozone-depleting substance emitted in the 21st century[J].Science,2009,326(5949):123-125.
[20]
IPCC.Climate change 2007:the physical science basis.Contribution of working group I to the fourth assessment report of the intergovernmental panel on climate change[M].Cambridge,United Kingdom,New York,NY,USA:Cambridge University Press,2007.
[21]
BURROWS R,NEVES M J V,LARSEN T,et al.European attitude to the disposal of wastewater in the sea:the case for a (European) network for sea outfall research[J].Water Science and Technology,1998,38(10):317-321.
[22]
COLLOS Y,MORNET F,SCIANDRA A,et al.An optical method for the rapid measurement of micromolar concentrations of nitrate in marine phytoplankton cultures[J].Journal of Applied Phycology,1999,11(2):179-184.
[23]
SCHALLER J L,ROYER T V,DAVID M B.Denitrification associated with plants and sediments in an agricultural stream[J].Journal of the North American Benthological Society,2004,23(4):667-676.
AMINOT A,KIRKWOOD D S,KÉROUEL R.Determination of ammonia in seawater by the indophenol-blue method:evaluation of the ICES NUTS I/C 5 questionnaire[J].Marine Chemistry,1997,56(1/2):59-75.
NEVISON C,BUTLER J H,ELKINS J W.Global distribution of N2O and theΔN2O-AOU yield in the subsurface ocean[J].Global Biogeochemical Cycles,2003,17(4),doi:10.1029/2003GB002068.
[43]
ROBERTS P J W,SNYDER W H.Hydraulic model study for Boston Outfall.II:environmental performance[J].Journal of Hydraulic Engineering,1993,119(9):988-1002.
[44]
GRACE R A.Marine outfall construction:background,techniques,and case studies[M].Washington,DC:American Society of Civil Engineers,2009.
[45]
BASTIAN R,WEBERLING R,PALILLA F.Ultraviolet spectrophotometric determination of nitrate...application to analysis of alkaline carbonates[J].Analytical Chemistry,1957,29(12):1795-1797.
SILVENNOINEN H,LIIKANEN A,TORSSONEN J,et al.Denitrification and nitrous oxide effluxes in boreal,eutrophic river sediments under increasing nitrate load:a laboratory microcosm study[J].Biogeochemistry,2008,91(2/3):105-116.
[49]
SPARROW S D,MASIAK D T.Errors inanalyses for ammonium and nitrate caused by contamination from filter papers[J].Soil Science Society of America Journal,1987,51(1):107-110.
KROEZE C,DUMONT E,SEITZINGER S P.New estimates of global emissions of N2O from rivers and estuaries[J].Environmental Sciences,2005,2(2/3):159-165.
[52]
WALTER R A.Mixing in Inland and Coastal waters by Hugo B.Fischer,E.John List; Robert C.Y.Koh; JÖrg Imberger; Norman H.Brooks[J].American Scientist,1980,68(6):692.
BUCK R P,SINGHADEJA S,ROGERS L B.Ultraviolet absorption spectra of some inorganic ions in aqueous solutions[J].Analytical Chemistry,1954,26(7):1240-1242.
[61]
RICHARDSON W B,STRAUSS E A,BARTSCH L A,et al.Denitrification in the Upper Mississippi River:rates,controls,and contribution to nitrate flux[J].Canadian Journal of Fisheries and Aquatic Sciences,2004,61(7):1102-1112.
[62]
DICKSON M L,WHEELER P A.Chlorophyll a concentrations in the North Pacific:does a latitudinal gradient exist?[J].Limnology and Oceanography,1993,38(8):1813-1818.
BANGE H W.Nitrous oxide and methane in European coastal waters[J].Estuarine,Coastal and Shelf Science,2006,70(3):361-374.
[65]
DUER M J.Use of variable orifice "duckbill" valves for hydraulic and dilution optimization of multiport diffusers[J].Water Science and Technology,1998,38(10):277-284.
[66]
KUANG C,LEE J H W.Numerical experiments on the mixing of a duckbill valve jet[C]//Proceedings of the 3rd International Symposium on Environmental Hydraulics.Phoenix,US:Arizona State University,2001:23-28.
OGURA N,HANYA T.Nature of ultra-violet absorption of sea water[J].Nature,1966,212(5063):758.
[72]
VAN KESSEL J F.Factors affecting the denitrification rate in two water-sediment systems[J].Water Research,1977,11(3):259-267.
[73]
KNEFELKAMP B,CARSTENS K,WILTSHIRE K H.Comparison of different filter types onchlorophyll-a retention and nutrient measurements[J].Journal of Experimental Marine Biology and Ecology,2007,345(1):61-70.
[74]
焦念志,杨燕辉.中国海原绿球藻研究[J].科学通报,2002,47(7):485-491.
[75]
RHEE T S,KETTLE A J,ANDREAE M O.Methane and nitrous oxide emissions from the ocean:a reassessment using basin-wide observations in the Atlantic[J].Journal of Geophysical Research:Atmospheres,2009,114(D12),doi:10.1029/2008JD011662.
[76]
GENTLE B S,ELLIS P S,GRACE M R,et al.Flow analysis methods for the direct ultra-violet spectrophotometric measurement of nitrate and total nitrogen in freshwaters[J].Analytica Chimica Acta,2011,704(1/2):116-122.
[77]
BONAGLIA S,NASCIMENTO F J A,BARTOLI M,et al.Meiofauna increases bacterial denitrification in marine sediments[J].Nature Communications,2014,5:5133.
[78]
BARNES J,UPSTILL-GODDARD R C.N2O seasonal distributions and air-sea exchange in UK estuaries:implications for the tropospheric N2O source from European coastal waters[J].Journal of Geophysical Research,2011,116(G1):G01006,doi:10.1029/2009JG001156.
[79]
ZHANG J Z,FISCHER C J.A simplified resorcinol method for direct spectrophotometric determination of nitrate in seawater[J].Marine Chemistry,2006,99(1/2/3/4):220-226.
STROUS M,HEIJNEN JJ,KUENEN J G,et al.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J].Applied Microbiology and Biotechnology,1998,50(5):589-596.
[88]
LIN H,DAI M H,KAO S J,et al.Spatiotemporal variability of nitrous oxide in a large eutrophic estuarine system:the Pearl River Estuary,China[J].Marine Chemistry,2016,182:14-24.
[89]
GRUBER N.The marine nitrogen cycle:overview and challenges[M]//Capone D G,BronkD A,Mulholland M R,et al.Nitrogen in the Marine Environment.2nd ed.San Diego:Academic Press,2008:1-50.
[90]
ZHU G B,JETTEN M S M,KUSCHK P,et al.Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems[J].Applied Microbiology and Biotechnology,2010,86(4):1043-1055.
[91]
CODISPOTI L A,CHRISTENSEN J P.Nitrification,denitrification and nitrous oxide cycling in the eastern tropical South Pacific Ocean[J].Marine Chemistry,1985,16(4):277-300.
[92]
ZHOU S,BORJIGIN S,RIYA S,et al.The relationship between anammox and denitrification in the sediment of an inland river[J].Science of the Total Environment,2014,490:1029-1036.
[93]
SANTORO A E,CASCIOTTI K L,FRANCIS C A.Activity,abundance and diversity of nitrifying archaea and bacteria in the central California Current[J].Environmental Microbiology,2010,12(7):1989-2006.
[94]
MEYER R L,RISGAARD-PETERSEN N,ALLEN D E.Correlation between anammox activity and microscale distribution of nitrite in a subtropical mangrove sediment[J].Applied and Environmental Microbiology,2005,71(10):6142-6149.
LÖSCHER C R,KOCK A,KÖNNEKE M,et al.Production of oceanic nitrous oxide by ammonia-oxidizing archaea[J].Biogeosciences,2012,9(7):2419-2429.
[97]
RICH J J,MYROLD D D.Community composition and activities of denitrifying bacteria from adjacent agricultural soil,riparian soil,and creek sediment in Oregon,USA[J].Soil Biology and Biochemistry,2004,36(9):1431-1441.
[98]
OSTROM N E,RUSS M E,POPP B,et al.Mechanisms of nitrous oxide production in the subtropical North Pacific based on determinations of the isotopic abundances of nitrous oxide and di-oxygen[J].Chemosphere-Global Change Science,2000,2(3/4):281-290.
[99]
MURRAY R E,PARSONS L L,SMITHM S,et al.Kinetics of nitrate utilization by mixed populations of denitrifying bacteria[J].Applied and Environmental Microbiology,1989,55(3):717-721.
[100]
NAQVI S W A,JAYAKUMAR D A,NARVEKAR P V,et al.Increased marine production of N2O due to intensifying anoxia on the Indian continental shelf[J].Nature,2000,408(6810):346-349.
[101]
GARCÍA-RUIZ R,PATTINSON S N,WHITTON B A.Kinetic parameters of denitrification in a river continuum[J].Applied and Environmental Microbiology,1998,64(7):2533-2538.
[102]
CABELLO P,ROLDÁN M D,MORENO-VIVIÁN C.Nitrate reduction and the nitrogen cycle in archaea[J].Microbiology,2004,150(11):3527-3546.
[103]
ZENG H,ZHANG T C.Evaluation of kinetic parameters of a sulfur-limestone autotrophic denitrification biofilm process[J].Water Research,2005,39(20):4941-4952.
[104]
BANGE H W,ANDREAE M O.Nitrous oxide in the deep waters of the world's oceans[J].Global Biogeochemical Cycles,1999,13(4):1127-1135.
FREING A,WALLACE D W R,BANGE H W.Global oceanic production of nitrous oxide[J].Philosophical Transactions of the Royal Society of London B:Biological Sciences,2012,367(1593):1245-1255.
[108]
MATěJ? V,?I?INSKÁ S,KREJ?ÍJ.Biological water denitrification-a review[J].Enzyme and Microbial Technology,1992,14(3):170-183.
[109]
CODISPOTI L A.Interesting times for marine N2O[J].Science,2010,327(5971):1339-1340.
[110]
SEITZINGER S P.Linkages between organic matter mineralization and denitrification in eight riparian wetlands[J].Biogeochemistry,1994,25(1):19-39.
[111]
KIM K R,CRAIG H.Two-isotope characterization of N2O in the Pacific Ocean and constraints on its origin in deep water[J].Nature,1990,347(6288):58-61.
[112]
STOCKENBERG A,JOHNSTONE R W.Benthic denitrification in the Gulf of Bothnia[J].Estuarine,Coastal and Shelf Science,1997,45(6):835-843.