Vertical profile of archaeal community in the sediments of Pearl River Mouth Basin in the Northeastern South China Sea
CHEN Ye1,2,3, ZHANG Yu2,3,4, ZHEN Yu2,3,4, MI Tie-zhu2,3,4, HE Hui1,2,3, WANG Xun-gong2,3,4
1. College of Marine Life Science, Ocean University of China, Qingdao 266003, China;
2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266100, China;
3. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
4. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China
Abstract：In this study, Illumina high-throughput sequencing technology and real-time quantitative PCR (RT-qPCR) were used to profile the archaeal community and vertical distribution in the surface, middle and bottom sediments of Pearl River Mouth Basin in the northeastern South China Sea. The results showed that the majority of archaeal sequences were classified into Thaumarchaeota, Woesearchaeota, Bathyarchaeota and Euryarchaeota. The archaeal community diversity and richness in the surface sediment were lowest in three samples, in which Marine Group I (MGI) was most abundant; in contrast, the main groups were MGI, Group C3, Marine Benthic Group B and Thermoplasmata in the middle and bottom sediments. Interestingly, Nitrosopumilus related to ammonia oxidation was dominant in the sediments, suggesting that nitrogen metabolism was probably active. RT-qPCR results illuminated the abundance of archaea ranging from 2.00×107 copies/g to 8.20×107 copies/g decreasing with the increasing depths. These results will provide a basis for further study of the archaeal community characteristics in the sediments of South China Sea.
CHEN Ye,ZHANG Yu,ZHEN Yu et al. Vertical profile of archaeal community in the sediments of Pearl River Mouth Basin in the Northeastern South China Sea[J]. Marine Environmental Science, 2017, 36(5): 735-740.
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