Physical-chemical factors associated with the dynamic change of bacterial quantity of alimentary tract and environment in Babylonia areolate industrial aquaculture
LING Hui1,2, ZHAO Wang1, WANG Rui-xuan1, WU Kai-chang1, WANG Jiang-yong1
1. Key Laboratory of Fishery Ecology and Environment, Guangzhou Province;Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, China Academy of Fishery Sciences, Guangzhou 510300, China;
2. College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China
Abstract：With the aims to master the dynamic change of bacteria quantity of alimentary tract and aquaculture environment in Babyloniaareolate, TCBS and nutrient AGAR cultivation method was applied to detection vibrio and heterotrophic bacteria quantity respectively, fluorescence microscopy was applied to detection water total bacteria (WTB). In addition, the main physical-chemical factors including temperature(T), salinity(S), pH, dissolved oxygen(DO) and major nutritive salt of water and sediment, were investigated. The results show that the quantity of WTB, water Vibrio(WV) and sediment Vibrio (SV) wave form 4.8×104~3.8×105 cells/mL, 2.1×102~1.2×104 CFU/mL and 6.0×104~1.9×106 CFU/g separately. Heterotrophic bacteria and Vibrio of Alimentary tract(AV) wave from 4.3×104~5.9×106 CFU/g and 2.0×102~7.5×105 CFU/g separately. Correlation analysis results show that the quantity of heterotrophic bacteria in Alimentary tract(AHB) and AV both are very significant positive correlation with nitrite (P<0.01), negative correlation with pH (P<0.05) and salinity (P<0.05). WTB is negative correlation with dissolved oxygen (P<0.05), WV is negative correlation with total phosphorus (P<0.05) and others are not obvious. Principal component analysis(PCA) show that WTB, WV, SV, as well as AHB and AV mainly affected by temperature, ammonia nitrogen and nitrite from September to the middle of November (autumn) while by water dissolved oxygen more in winter. In particularly, salinity and pH should be monitoring during the whole cultivation period.
LING Hui,ZHAO Wang,WANG Rui-xuan et al. Physical-chemical factors associated with the dynamic change of bacterial quantity of alimentary tract and environment in Babylonia areolate industrial aquaculture[J]. Marine Environmental Science, 2018, 37(1): 62-69.
DEHLER CE,SECOMBES CJ,MARTIN SAM.Environmental and physiological factors shape the gut microbiota of Atlantic salmonparr (Salmosalar L.)[J].Aquaculture,2017,467:149-157.
[4]
INGERSLEV HC,STRUBE ML,VON GERSDORFF JØRGENSEN L,et al.Diet type dictates the gut microbiota and the immune response against Yersinia ruckeri in rainbow trout (Oncorhynchusmykiss)[J].Fish &Shellfish Immunology,2014,40(2):624-633.
[5]
BENSON A K,KELLY S A,LEGGE R,et al.Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(44):18933-18938.
[6]
REVECO F E,ØVERLAND M,ROMARHEIM O H,et al.Intestinal bacterial community structure differs between healthy and inflamed intestines in Atlantic salmon (Salmosalar L.)[J].Aquaculture,2014,420/421:262-269.
CAO H P,AN J,ZHENG W D,et al.Vibrio cholerae pathogen from the freshwater-cultured whiteleg shrimp Penaeusvannamei and control with Bdellovibriobacteriovorus[J].Journal of Invertebrate Pathology,2015,130:13-20.
LÓPEZ-HERNÁNDEZ K M,PARDÍO-SEDAS V T,LIZÁRRAGA-PARTIDA L,et al.Environmental parameters influence on the dynamics of total and pathogenic Vibrio parahaemolyticus densities in Crassostreavirginica harvested from Mexico's Gulf coast[J].Marine Pollution Bulletin,2015,91(1):317-329.
[11]
MERLO C,REYNA L,ABRIL A,et al.Environmental factors associated with heterotrophic nitrogen-fixing bacteria in water,sediment,and riparian soil of Suquía River[J].Limnologica-Ecology and Management of Inland Waters,2014,48:71-79.
[12]
XIONG JB,WANG K,WU JF,et al.Changes in intestinal bacterial communities are closely associated with shrimp disease severity[J].Applied Microbiology and Biotechnology,2015,99(16):6911-6919.
AUGUET J C,MONTANIÉ H,DELMAS D,et al.Dynamic of virioplankton abundance and its environmental control in the Charente estuary (France)[J].Microbial Ecology,2005,50(3):337-349.
[18]
BETTAREL Y,BOUVY M,DUMONT C,et al.Virus-bacterium interactions in water and sediment of West African inland aquatic systems[J].Applied and Environmental Microbiology,2006,72(8):5274-5282.
YU W T,JONG K J,LIN Y R,et al.Prevalence of Vibrio parahaemolyticus in oyster and clam culturing environments in Taiwan[J].International Journal of Food Microbiology,2013,160(3):185-192.
