王志富, 丰卫华, 许恒韬, 陈悦, 郑芳琴, 陈立红. 不同无机氮对锥状斯氏藻生长和抗氧化酶活性的影响[J]. 海洋环境科学, 2018, 37(2): 221-227. DOI: 10.12111/j.cnki.mes20180210
引用本文: 王志富, 丰卫华, 许恒韬, 陈悦, 郑芳琴, 陈立红. 不同无机氮对锥状斯氏藻生长和抗氧化酶活性的影响[J]. 海洋环境科学, 2018, 37(2): 221-227. DOI: 10.12111/j.cnki.mes20180210
WANG Zhi-fu, FENG Wei-hua, XU Heng-tao, CHEN Yue, ZHENG Fang-qin, CHEN Li-hong. Effects of different nitrogen source on antioxidant enzymes activities of Scrippsilla trochodiea[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2018, 37(2): 221-227. DOI: 10.12111/j.cnki.mes20180210
Citation: WANG Zhi-fu, FENG Wei-hua, XU Heng-tao, CHEN Yue, ZHENG Fang-qin, CHEN Li-hong. Effects of different nitrogen source on antioxidant enzymes activities of Scrippsilla trochodiea[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2018, 37(2): 221-227. DOI: 10.12111/j.cnki.mes20180210

不同无机氮对锥状斯氏藻生长和抗氧化酶活性的影响

Effects of different nitrogen source on antioxidant enzymes activities of Scrippsilla trochodiea

  • 摘要: 本文研究了不同浓度的无机氮对锥状斯氏藻(Scrippsiella trochoidea)生长和抗氧化酶活性的影响。结果显示,NO3-N更适于锥状斯氏藻的生长,而NH4-N更容易被藻细胞吸收。两种不同氮源对于锥状斯氏藻单位细胞内抗氧化酶活性的影响都表现为随着氮浓度升高而降低。但不同种氮源的影响在不同氮浓度、不同生长期时有差异,如90 μM的NH4-N在藻类的指数生长期能促进抗氧化酶活性,10 μM的NH4-N在藻类生长的平台期期能促进抗氧化酶活性,而相同浓度的NO3-N没有这种作用,其他生长期时两种氮源对抗氧化酶活性的影响差别不大。这可能和锥状斯氏藻对NH4-N的优先利用有关。

     

    Abstract: The article is to explore the effects of inorganic nitrogen with various concentrations to the growth and cyst of Scrippsiella trochoidea.The study indicates NO3-N is favorable to cell growth, while NH4-N is preferable to absorb the vegetative cells.The antioxidant enzyme activities decreased along with rising concentration of nitrogen.But the different nitrogen source had different effects when the concentration and growth period were different.For example, compared with NO3-N, 90 μM NH4-N could promote the antioxidant enzyme activities in the phases of exponential growth, and 10 μM NH4-N could promote the antioxidant enzyme activities in the phases of plateau.For other periods, these two nitrogen sources have less influence, which could be attributed to the prior absorption of NH4-N.

     

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