Abstract: Based on the trial guidelines (2016 edition) to construct a monitoring and forecasting technology and method for resources and environment carrying capacity, combining the distinctive attribute of fishery resources and the practical situation of Dongshan bay, an assessment indices system and method for fishery resources carrying capacity in Dongshan bay has been modified. Based on the investigation on nekton, fish eggs, fish larvae and juveniles in the spring (April) and autumn (November) of 2016, combining the investigation data from 2012 to 2013 to make comprehensive assessments. The result showed that the fishery resources carrying situation in Dongshan bay has been critical. Therefore, we proposed forewarning measures, such as further strengthen monitoring survey of fisheries resources, ecological environmental protection and supervision and management, in order to provide the theory basis for sustainable utilization of fishery resources in Dongshan bay, furthermore, to provide reference and technical support for further promoting the operational marine resources bearing capacity assessment and fishery resources carrying capacity evaluation of other bays.