袁蕾, 张纯超, 吕彦儒. 海水中溶解氨基酸分析技术的研究进展[J]. 海洋环境科学, 2020, 39(6): 960-967. DOI: 10.12111/j.mes.20190172
引用本文: 袁蕾, 张纯超, 吕彦儒. 海水中溶解氨基酸分析技术的研究进展[J]. 海洋环境科学, 2020, 39(6): 960-967. DOI: 10.12111/j.mes.20190172
YUAN Lei, ZHANG Chun-chao, LV Yan-ru. Advances in analytical technique research for dissolved amino acids in seawater[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2020, 39(6): 960-967. DOI: 10.12111/j.mes.20190172
Citation: YUAN Lei, ZHANG Chun-chao, LV Yan-ru. Advances in analytical technique research for dissolved amino acids in seawater[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2020, 39(6): 960-967. DOI: 10.12111/j.mes.20190172

海水中溶解氨基酸分析技术的研究进展

Advances in analytical technique research for dissolved amino acids in seawater

  • 摘要: 随着对海洋中有机氮生物地球化学循环过程认识的逐步深入,氨基酸作为海洋有机氮库中最易被生物利用的组分之一愈加受到重视。关于海洋中氨基酸含量组成和生物可利用性的相关研究对认识海洋中有机氮的迁移和转化具有重要意义。本文详细阐述了海洋领域分光法和荧光法等溶解氨基酸测定方法及离子色谱和液相色谱等色谱分离方法的发展历程,并对应用最普遍的柱前衍生-反相液相色谱-荧光检测法等技术进行了更为详细的评述,列举了质谱技术、单体同位素分析技术和毛细管电泳等最新发展的技术方法,最后对其在海洋学中的应用进行展望。

     

    Abstract: Amino acids, as one of the most important readily bioavailable components in marine organic nitrogen pool, have attracted more and more attention with the deepening understanding of the biogeochemical cycle of organic nitrogen in the marine environment. Studies on composition and bioavailability of amino acids are of great significance to understand the migration and transformation of organic nitrogen in the ocean. In this paper, the advances in analytical methods for dissolved amino acids in seawater, such as spectrophotometry and fluorospectrophotometry and separation methods, such as ion exchange chromatography (IEC) and high-performance liquid chromatography (HPLC) are detailed explained. And the most widely used precolumn derivatives-reversed-phase liquid chromatography-fluorescence detection method is more detailed reviewed. Moreover, the latest techniques such as mass spectrometry (MS), compound-specific isotope analysis (CSIA) and capillary electrophoresis (CE) are also mentioned, as well as their prospected applications in oceanography.

     

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