Abstract: Tetracycline（TC）has been widely used in the marine aquaculture industry. However, overdosing TC can lead to the residue that is difficult to be removed by conventional methods in the tail water. This paper used catalytic ozone oxidation technology to degrade TC in simulated marine aquaculture tail water, and analyzed the data in multi-angle to prove the feasibility of this technology. Results of the experiment showed as follow: in the catalytic ozone reaction system that using A-Mn/CeO-γAl₂O₃ as the catalyst, the TC removal rate increased with the increase dosage of ozone and catalyst. Ozone 4.46 mg/L, catalyst 186.67 g and reaction time not less than 3.27 minutes were the optimum reaction conditions. The removal rate of TOC in simulated tail water was 71.6% after 1 hour reaction. The organics of humic acid and fulvic acid could be quickly removed by the reaction system. TC was transformed into CO₂ and H₂O after reactions including molecular rearrangement in the reaction system. TC was transformed into CO₂ and H₂O, and the biological toxicity of simulated tail water was reduced after reaction.