Abstract: The effects of different temperatures (29 ℃, 31 ℃, 33 ℃, 35 ℃ and 37 ℃) on the synchronization of molting, body immunity and oxidation resistance of juvenile Chinese horseshoe crab (Tachypleus tridentatus) were explored. The results showed that: (1) The survival rate decreased significantly when the temperature was higher than 33 ℃ (P<0.05). With the increase of temperature, the molting weight gain rate decreased significantly (P<0.05). The molting rate increased significantly at first and then decreased significantly with the increase of temperature (P<0.05) , and the 33 ℃ group was the highest. (2) The molting time of higher temperature test groups were earlier, and the 33 ℃ group was the first. The emergy of the two-stage juvenile horseshoe crabs tended to decrease with the increasing temperature. (3) The ACP activity of the horseshoe crabs at 37 ℃ was significantly lower than that of other groups (P<0.05), while the AKP activity had no obvious regularity.(4)With the increasing temperature, the CAT activities of the first-year-old horseshoe crabs significantly decreased. And the T-AOC and CAT activities of the 37 ℃ test group were significantly lower than those of other test groups (P<0.05). The results showed that when the temperature was lower than 33 ℃, raising the temperature properly was conducive to promote the molting and growth of juvenile horseshoe crabs. After exceeding 33 ℃, the immunity and oxidation resistance of juvenile horseshoe crabs decreased significantly, and the survival rate decreased. This study provides a theoretical basis for the artificial breeding and releasing of Chinese horseshoe crab.