红色中缢虫赤潮中浮游植物细胞碱性磷酸酶的研究
The study on alkaline phosphatase activity (APA) of phytoplanktons in the bloom of Mesodinium rubrum based on ELF@97
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摘要: 2009年12月14日,在对球形棕囊藻Phaeocystis globosa赤潮的跟踪调查过程中发现一次红色中缢虫赤潮,设置3个站位对红色中缢虫赤潮进行调查监测。利用酶标记荧光(enzyme labeled fluorescence,ELF@97)方法,对浮游植物碱性磷酸酶进行标记,研究红色中缢虫赤潮发生时,单个浮游植物碱性磷酸酶活性特征。得出以下结论:(1)浮游植物碱性磷酸酶总体的标记率达51%,其中甲藻的碱性磷酸酶具有很强的表达,标记率超过99%;硅藻的碱性磷酸酶表达很弱,标记率仅为3.4%。(2)甲藻比硅藻很容易被诱导产生碱性磷酸酶。(3)浮游植物在与红色中缢虫营养盐竞争中处于劣势。Abstract: A bloom of Mesodinium rubrum was found in the investigation of the bloom of Phaeocystis globosa on December 14th, 2009.Then, three sampling stations were set up for monitoring the bloom of Mesodinium rubrum. During the bloom of Mesodinium rubrum, the alkaline phosphatase activity of a single phytoplankton was characterized using the methodology of enzyme-labeled fluorescence.It was found that:(1) the overall labeled rate of alkaline phosphatase activity in phytoplankton was 51%, in which dinoflagellate had a strong expression of alkaline phosphatase with a labeled rate higher than 99%; while diatoms had a very weak expression of alkaline phosphatase with a labeled rate of only 3.4%; (2) dinoflagellate was easier to be induced to produce alkaline phosphatase with respect to diatoms; (3) phytoplankton was at a disadvantage in competing for nutrients, compared to Mesodinium rubrum.
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