Abstract: Safety and healthy evaluation of the shellfish flesh and waters in the mariculture zones is significantly important for human health risk assessment and effective management.So we collected shellfish and seawater from Jinshitan, Dalijia and Dachangshandao mariculture zones of Dalian in March, May, August and October of 2013.And we analyzed the total coliform(TC), fecal coliform(FC) in them.At the same time, the total vibrios in seawater and environmental factors including temperature, pH, salinity, COD, dissolved oxygen and chlorophyll a were also evaluated.The results showed that:the concentration of TC and FC changed with the spatial and temporal distribution distribution.The temporal distribution of TC concentration in shellfish tissues was August > May > Month > October, and the spatial distribution was Dachangshandao > Jinshitan, Dachangshandao > Dalijia which has no significant difference between Jinshitan and Dalijia (P < 0.05).The distribution characteristics of TC and FC in seawater was similar.The temporal distribution was:the highest TC and FC concentrations in seawater were August for Jinshitan and May for Dalijia, however, the TC and FC concentrations in seawater were less than 2 MPN/100 mL in Dachangshandao during the sampling period, and the spatial distribution of TC and FC concentration in seawater was highest in Dalijia and there's no significant difference between Jinshitan and Dachangshandao.The TV concentration in water were higher in August and October which ranged from 3.3×10² to 4.23×10⁵ CFU/100 mL.By analyzing the correlation between TC、FC、TV and their environmental factors (temperature, pH, salinity, COD, dissolved oxygen and chlorophyll a) in the three mariculture zones and different seasons, we can know that environmental factors and the characteristics of the mariculture zones have important effects on the temporal and spatial distribution of fecal indicator bacteria and vibrios.In order to better reflect the health and safety status of the mariculture area, the indicator bacteria in the seawater and shellfish should be monitored simultaneously.In addition we recommended vibrios as an important pathogen indicator, so we should monitor it at the same time as traditional indicator bacteria.