Abstract: To establish a rapid detection method based on colloidal gold immune chromatography assay for detection okadaic acid and its derivatives(Dinophysistoxins) in marine bivalves.Appropriate concentration of coupling antigen OA-OVA and HRP marked goat anti mouse IgG were coated as the test line and control line respectively, colloidal gold labeling anti-OA monoclonal antibody coated as the conjugate pad, method detection limit was improved by optimize the coating concentration.The results showed that the detection of limit of the strip was 16 ng/mL(OA, DTX1 & DTX2), detection time was 10min.The established methods in present study for detection of okadaic acid and its derivatives can fufill the general regulation that OA in shellfish must be inferior to the safe threshold (160 μg/kg), and this method can be used to semi-quantitative assessment of the shellfish samples that contaminated diarrhetic shellfish poisoning.