Distribution characteristics of antibiotic resistance bacteria and related resistance genes in mariculture area of Shandong

Antibiotic resistance bacteria and related resistance genes in five mariculture areas of Shandong were detected,and in both five samples it was found that the percentage of tetracycline-,Sulfonamides- and -lactam-resistant bacteria were significantly (p0.05) higher than those chloromycetin- and quinolone-resistant bacteria.The abundance of 15 antimicrobial resistant genes was determined by RT-PCR methods.It was obtained that the abundance of sulfonamides-(sul,dfra/16S rRNA=10-6~10-2),quinolone-(qnr/16S rRNA=10-6~10-2),and tetracycline-resistance genes (tet/16S rRNA=10-7~10-2) has no significant differences among the five water samples,but the abundance of chloramphenicol-resistant genes (cata,cmle/16S rRNA=10-8~10-2) has significant differences among the five water samples and the abundance of the cata1 and cmle1 has significant correction (p0.05) with the percentage of chloramphenicol-resistant bacteria.All the results indicated the emergence pollution of antimicrobial resistant bacteria and related resistance genes in mariculture environments of Shandong,China.