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Volume 34 Issue 1
Jan.  2015
Article Contents

Citation:

Identification of several fish eggs and larvae by DNA barcoding in Xiamen Water

  • Received Date: 2014-08-18
    Accepted Date: 2014-11-04
  • This research focus on identification of fish eggs and larvae collected in Xiamen waters. The samples were distinguished to 2 types of fish eggs and 3 types of fish larvae by morphological characters and were identified as 3 species fish eggs and 4 species fish larvae by DNA barcoding technology. In which, there were six species and one genus were identified, including Thryssa kammalensis egg, Nuchequula nuchalis egg, Tridentiger trigonocephalus larva, Tridentiger barbatus larva, Konosirus punctatus larva, Platycephalus indicus larva and Ambassis sp. egg. The results showed that molecular technology was necessary in identification of fish eggs and larvae to species, especially those species without enough morphological characters. By using automation and standardization system, DNA barcoding technology can identify the species of fish eggs and larvae effectively. This technology with the advantages of high reliability, excellent repetition and less error is a powerful complement for traditional identification by morphology and has wide application prospects in the study of ichthyoplankton diversity.
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  • [1] 郑光明,朱新平,张 跃,等.RAPD技术鉴定3种鲮的研究[J].华中农业大学学报,1999,18 (4): 371-374.[5]刘光明,史千玉,曹敏杰,等.利用PCR和限制性酶切技术鉴别3种鳗鱼[J].集美大学学报(自然科学版),2011,16 (3): 179-181.[9]柯慧玲.南台湾垦丁周边海域仔鱼群聚之时空分布及DNA生命条码在鉴种上之应用[D].台湾: 海洋生物研究所,2007.[10]卞晓东,张秀梅,高天翔,等.沙氏下鱵鱼卵的形态学及遗传学鉴别[J].水产学报,2008,32 (3): 342-352.[11]张继民,刘 霜,赵建民,等.带鱼(Trichiurus haumela)鱼卵DNA的提取及其18S rDNA初步分析[J].海洋通报,2009,28 (6): 62-65.[12]张远辉,陈国辉.厦门海域二氧化碳及其生物生产力的估算[J].海洋通报,2000,19 (2): 30-35.[13]黄宗国.厦门湾物种多样性[M].北京: 海洋出版社,2006,5-6.[14]黄良敏,谢仰杰,李 军,等.厦门海域鱼类群落分类学多样性的研究[J].海洋学报,2013,35 (2): 126-132.[15]蔡秉及,王志远.厦门港及邻近海域的浮性鱼卵和仔、稚鱼[J].台湾海峡,1994,13 (2): 204-208.[16]江素菲,陈 枫.九龙江口鱼类浮游生物的生态[J].台湾海峡,1993,12 (4): 351-358.[17]林 楠,沈长春,钟俊生.九龙江口沿岸碎波带仔稚鱼种类组成[J].上海海洋大学学报,2009a,18 (6): 686-694.[18]林 楠,沈长春,钟俊生.九龙江口仔、稚鱼种类组成和季节变化[J].南方水产,2009b,5 (4): 1-8.[20]邵广昭,杨瑞森,陈康青,等.台湾海域鱼卵图鉴[M].台北: 中央研究院动物研究所,2001.[21]冲山宗雄.日本产稚鱼图鉴[M].东京: 东京大学出版会,1988.[22]张孝威,陈真然,阮洪超,等.赤鼻棱鳀、中颌棱鳀卵子、仔稚鱼的发育[J].动物学报,1982,28 (2): 183-189.[23]陈真然,张孝威.斑鰶卵子和仔、稚、幼鱼的形态特征[J].海洋与湖沼,1965,7 (3): 205-214.[24]张孝威,沙学绅,何桂芬,等.鯒鱼卵子和仔、稚鱼的形态观察[J].海洋与湖沼,1980,11 (2): 161-168.[32]ZHANG J.Species Identification of Marine Fishes in China with DNA Barcoding[J].Evidence-Based Complementary and Alternative Medicine,2011(2),online doi:10.1155/2011/978253.[34]伍汉霖,邵广昭,赖春福.拉汉世界鱼类名典[M].台湾: 水产出版社,1999.
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Identification of several fish eggs and larvae by DNA barcoding in Xiamen Water

  • 1. College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, China;
  • 2. Guangxi Mangrove Research Center, Beihai 536007, China

Abstract: This research focus on identification of fish eggs and larvae collected in Xiamen waters. The samples were distinguished to 2 types of fish eggs and 3 types of fish larvae by morphological characters and were identified as 3 species fish eggs and 4 species fish larvae by DNA barcoding technology. In which, there were six species and one genus were identified, including Thryssa kammalensis egg, Nuchequula nuchalis egg, Tridentiger trigonocephalus larva, Tridentiger barbatus larva, Konosirus punctatus larva, Platycephalus indicus larva and Ambassis sp. egg. The results showed that molecular technology was necessary in identification of fish eggs and larvae to species, especially those species without enough morphological characters. By using automation and standardization system, DNA barcoding technology can identify the species of fish eggs and larvae effectively. This technology with the advantages of high reliability, excellent repetition and less error is a powerful complement for traditional identification by morphology and has wide application prospects in the study of ichthyoplankton diversity.

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