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Volume 34 Issue 4
Jul.  2015
Article Contents

Citation:

The preliminary study of single-cell PCR analysis of marine Dinoflagellates

  • Received Date: 2014-04-09
    Accepted Date: 2014-07-04
  • Dinoflagellates is one of the important harmful algae bloom forming species.Because of the similarity in size and shape,micro-dinoflagellates were difficult to be identified exactly using morphological approaches.Single-cell PCR is probably the most rapid and convenient method that can identify marine dinoflagellates accurately.In this study,Alexandrium tarmarense were fixed with glutaraldehyde,Lugol's solution and formalin respectively.Before undertaken single-cell PCR,the whole genome of single cell was amplified.The results showed that the cells preserved in glutaraldehyde and Lugol's solution could be analyzed using sing-cell PCR in 30 days.While there were negative results in the A.tarmarense cells preserved in formalin.The dinoflagellate Peridinium conicum collected in Lingshui Bay in Dalian were fixed with glutaraldehyde at final concentration of 2%,and successfully identified by combining light microscopy and single cell PCR Amplification methods.
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  • [1] 周名江,朱明远,张 经.中国赤潮的发生趋势和研究进展[J].生命科学,2001,13(2):54-59.[13]陈月琴,屈良鹄,邱小忠,等.甲藻单个细胞DNA的制备及在赤潮藻类分子鉴定中的应用[J].中山大学学报:自然科学版,1997,36(4):66-69.[14]李 萍,于仁成,唐祥海,等.用于单一藻细胞PCR扩增反应的样品保存方法比较[J].海洋科学,2008,32(6):41-45.[15]李 萍.应用单细胞PCR方法研究青岛近海一株鳍藻的遗传特征[D].青岛:中国科学院研究生院 (海洋研究所),2007.[16]唐祥海,于仁成,张清春,等.应用单细胞rDNA序列测定方法鉴定不同生活史阶段的亚历山大藻[J].高技术通讯,2008,18(11):30-35.[18] GB12763-2007,海洋调查规范[S].
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The preliminary study of single-cell PCR analysis of marine Dinoflagellates

  • 1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
  • 2. National Marine Environmental Monitoring Center, Dalian 116023, China

Abstract: Dinoflagellates is one of the important harmful algae bloom forming species.Because of the similarity in size and shape,micro-dinoflagellates were difficult to be identified exactly using morphological approaches.Single-cell PCR is probably the most rapid and convenient method that can identify marine dinoflagellates accurately.In this study,Alexandrium tarmarense were fixed with glutaraldehyde,Lugol's solution and formalin respectively.Before undertaken single-cell PCR,the whole genome of single cell was amplified.The results showed that the cells preserved in glutaraldehyde and Lugol's solution could be analyzed using sing-cell PCR in 30 days.While there were negative results in the A.tarmarense cells preserved in formalin.The dinoflagellate Peridinium conicum collected in Lingshui Bay in Dalian were fixed with glutaraldehyde at final concentration of 2%,and successfully identified by combining light microscopy and single cell PCR Amplification methods.

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